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Schematic comparing RT-PCR, qPCR and RT-qPCR. yiruma stay in memory (A) RT-PCR workflow RNA is isolated and cDNA is generated via reverse transcription (RT); PCR is then carried out to amplify areas of interest (B) qPCR schematic DNA is isolated and amplified; amplification is quantitated using a probe which fluoresces upon intercalation with double-stranded DNA